-
Hoechst 33342
Hoechst 33342
- 中文名称：
- Hoechst 33342
- 英文名称：
- Hoechst 33342
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA332503
- 规格：
- 10 mg|100 mg|2x100 mg|3x100 mg|4x100 mg
- 保存建议：
- Store at -20 degree C and protect from light.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Hoechst 33342, also known as bis Benzimide H 33342 or HOE 33342, DAPI has the molecular formula C27H28N6O-3HCl-3H2O, molecular weight 616.0 and CAS number 881691-13-0, is a non-embedded bright blue fluorescent dye. Dyes have weak fluorescence in solution, but they become bright after binding to DNA in the groove of DNA poly AT sequence enriched region in living cells, so such dyes are also called DNA probes. Due to the low background staining cells do not need washing and the staining is very stable, non-toxic to living cells, and the fluorescence can last for several days or longer after combining with DNA staining. Hoechst33342 has lower solubility in water than Hoechst 33258, but both dyes have high cell membrane permeability and are widely used for apoptosis detection. After staining, fluorescence microscopy or flow cytometry can be used for detection. In the case of adherent cells (96-well plates), 100 uL of the staining solution was used in each well, and the concentration of the staining solution was calculated as 5 ug/mL. 10 mg of the staining solution could be used in 20,000 wells.
