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Luminescent Mycoplasma Detection Kit
Luminescent Mycoplasma Detection Kit
- 中文名称：
- Luminescent Mycoplasma Detection Kit
- 英文名称：
- Luminescent Mycoplasma Detection Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA332629
- 规格：
- 20 Tests|200 Tests|2x200 Tests|3x200 Tests|4x200 Tests
- 保存建议：
- Stored at -20 degree C for 12 months, protect from light
All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Liquid solution
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
The Luminescent Mycoplasma Detection Kit utilizes Mycoplasma-specific kinase activity and detects it through a luminescent reaction catalyzed by ATP-dependent luciferase, and determines whether a sample is contaminated with Mycoplasma by comparing the change in ATP content before and after addition of the test reagent. When Mycoplasma is cleaved, it releases a unique kinase that reacts with the substrate and catalyzes the conversion of ADP to ATP.
Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8um, some mycoplasma can pass 0.22 um filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition.
Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important.
