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Micro D-lactate Dehydrogenase (D-LDH) Activity Assay Kit
Micro D-lactate Dehydrogenase (D-LDH) Activity Assay Kit
- 中文名称:
- Micro D-lactate Dehydrogenase (D-LDH) Activity Assay Kit
- 英文名称:
- Micro D-lactate Dehydrogenase (D-LDH) Activity Assay Kit
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA332633
- 规格:
- 48 Tests|96 Tests|2x96 Tests|3x96 Tests|4x96 Tests
- 保存建议:
- Stored at -20 degree C for 6 months.
- 货期:
- 6-8周
- 纯度:
- N/A
- 产品形式:
- N/A
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Principle of the Assay: Lactate dehydrogenase (LDH) is a glycolytic enzyme, which widely exists in animals, plants, microorganisms and cultured cells, with a higher content in kidney. LDH is the terminal enzyme of the glycolytic pathway, catalyzing the reversible reaction between pyruvate and lactate, accompanied by the interconversion between NAD+/NADH. According to the different configuration of its catalytic substrate lactate, it can be divided into D-lactate dehydrogenase (D-LDH, EC 1.1.1.28) and L-lactate dehydrogenase (L-LDH, EC 1.1.1.27). D-LDH catalyzes NAD+ to oxidize D-lactic acid to produce pyruvate. Pyruvate further reacts with 2,4-dinitrophenylhydrazine to produce pyruvate dinitrophenylhydrazone, which appears brownish red in alkaline solution, and the color depth is proportional to the concentration of pyruvate.