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DH5-alpha Competent Cells
DH5-alpha Competent Cells
- 中文名称:
- DH5-alpha Competent Cells
- 英文名称:
- DH5-alpha Competent Cells
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA37867
- 规格:
- 1 Each|2x1 Each|3x1 Each|5x1 Each|4x1 Each
- 保存建议:
- Store at -70°C
Shipping: Dry Ice
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
- 货期:
- 6-8周
- 纯度:
- N/A
- 产品形式:
- Frozen liquid
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
DH5-alpha strain chemically competent cells from the lab are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate. For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.
Genotype: F-phi80lacZdeltaM15 delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 lambda-