-
Recombinant Synaptosomal-associated protein 25kDa, C.elegans
Recombinant Synaptosomal-associated protein 25kDa, C.elegans
- 中文名称：
- Recombinant Synaptosomal-associated protein 25kDa, C.elegans
- 英文名称：
- Recombinant Synaptosomal-associated protein 25kDa, C.elegans
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA38472
- 规格：
- 0.01 mg|0.05 mg|1 mg|2x1 mg|3x1 mg
- 保存建议：
- Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
- 货期：
- 6-8周
- 纯度：
- Greater than 95.0% as determined by SDS-PAGE.
- 产品形式：
- The protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT, 1mM EDTA and 10% Glycerol.
Sterile filtered colorless solution.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: Recombinant C.elegans SNAP-25 produced in E Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E Coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
Introduction: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters.
