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Recombinant Tobacco Etch Virus Protease
Recombinant Tobacco Etch Virus Protease
- 中文名称:
- Recombinant Tobacco Etch Virus Protease
- 英文名称:
- Recombinant Tobacco Etch Virus Protease
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA38573
- 规格:
- 100 IU|500 IU|1000 IU|2x1000 IU|3x1000 IU
- 保存建议:
- rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
- 货期:
- 6-8周
- 纯度:
- Greater than 90.0% as determined by analysis by SDS-PAGE.
- 产品形式:
- The rTEV contains 25mM Tris, 75mM NaCI, 5mM EDTA, 10mM GSH, 50% Glycerol.
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
Description: Recombinant TEV Protease (rTEV) is a site-specific protease purified from E Coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30 degree C; however, the enzyme can be used at temperatures as low as 4 degree C. The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques.
Introduction: TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.