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Recombinant Human O-6-Methylguanine-DNA Methyltransferase
Recombinant Human O-6-Methylguanine-DNA Methyltransferase
- 中文名称：
- Recombinant Human O-6-Methylguanine-DNA Methyltransferase
- 英文名称：
- Recombinant Human O-6-Methylguanine-DNA Methyltransferase
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA38775
- 规格：
- 0.005 mg|0.02 mg|1 mg|2x1 mg|3x1 mg
- 保存建议：
- Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommeded to add a carrier protein (0.1% HSA or BSA)
Avoid multiple freeze-thaw cycles.
- 货期：
- 6-8周
- 纯度：
- Greater than 95.0% as determined by SDS-PAGE.
- 产品形式：
- The MGMT solution (0.5 mg/ml) contains 20mM Tris-HCl pH-7.5, 1mM DTT and 10% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: MGMT Human Recombinant fused to a 20 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 227 amino acids (1-207) and having a molecular mass of 23.8 kDa. The MGMT is purified by proprietary chromatographic techniques.
Introduction: MGMT is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and takes part in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. abnormal MGMT expression correlates with the prognosis in human solid cancers. MGMT decrease of expression is correlated with methylation. The human MGMT is a negative regulator of estrogen receptor-mediated transcription upon alkylation DNA damage. MGMT promoter hypermethylation plays an important role in the early steps of colorectal carcinogenesis. Abnormal promoter hypermethylation of MGMT gene is associated with oral squamous cell carcinomas.
