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Recombinant Salmonella Enteritidis Outer Membrane Protein-A
Recombinant Salmonella Enteritidis Outer Membrane Protein-A
- 中文名称：
- Recombinant Salmonella Enteritidis Outer Membrane Protein-A
- 英文名称：
- Recombinant Salmonella Enteritidis Outer Membrane Protein-A
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA39040
- 规格：
- 0.1 mg|0.5 mg|1 mg|2x1 mg|3x1 mg
- 保存建议：
- OmpA S. Enteritidis Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
- 货期：
- 6-8周
- 纯度：
- Protein is >95% pure as determined by 10% PAGE (coomassie staining).
- 产品形式：
- 1.77 mg/mL in PBS and 25mM Arginine.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: The Recombinant Salmonella Enteritidis Outer Membrane Protein A, E Coli derived, 330 amino acids, contains the ompA immunodominant regions. The protein is fused to a His tag at C-terminal and purified by standard chromatography techniques.
Introduction: The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A.salmonicida, Shigella dysenteriae and E.coli.OmpA's major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity, as well as a role in conjugation and bacteriophage binding.Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease.Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein.The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E.coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein.Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A.salmonicida bacterial cells by the glycine and the membrane extraction methods.
