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StemTAG Alkaline Phosphatase Staining Kit (Purple)
StemTAG Alkaline Phosphatase Staining Kit (Purple)
- 中文名称：
- StemTAG Alkaline Phosphatase Staining Kit (Purple)
- 英文名称：
- StemTAG Alkaline Phosphatase Staining Kit (Purple)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA44605
- 规格：
- 100 Assays|2x100 Assays|3x100 Assays|4x100 Assays|5x100 Assays
- 保存建议：
- Store all components at 4 degree C until their expiration dates
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: Embryonic stem (ES) cells are continuous proliferating stem cell lines of embryonic origin first isolated from the inner cell mass (ICM). Two distinguishing features of ES cells are their ability to be maintained indefinitely in an undifferentiated state and their potential to develop into any cell within the body. Based on previous methods developed for mouse ES cells, human ES cell lines were first established by Dr. James Thomson and colleagues. Like mouse ES cells, human ES cells express high levels of membrane alkaline phosphatase (AP) and Oct-4, a transcriptional factor critical to ICM and germline formation. However, unlike mouse ES cells, hES cells do not express stage-specific embryonic antigen (SSEA-1). In addition, prolonged propagation of hES cells is typically achieved by coculture with primary mouse embryonic fibroblasts (MEFs) serving as feeder cells. Human ES cell lines are not able to maintain their undifferentiated state in the absence of supporting feeder layer cells, even when exogenous cytokines such as leukemia inhibitory factor (LIF) and gelatin-coated plates are used. Although stem cells from different origins require different growth conditions for self-renewal and display different cell surface markers (see Table 1), AP is the most widely used stem cell marker. The StemTAG Alkaline Phosphatase Staining Kit provides an efficient system for monitoring ES cell undifferentiation/ differentiation through AP activity by immunocytochemistry staining.
