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293RTV Cell Line
293RTV Cell Line
- 中文名称：
- 293RTV Cell Line
- 英文名称：
- 293RTV Cell Line
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA44655
- 规格：
- 1 Vial|2x1 Vial|3x1 Vial|4x1 Vial|5x1 Vial
- 保存建议：
- Liquid nitrogen
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80ºC until first culture. Store subsequent cultured cells long term in liquid nitrogen.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
The 293RTV Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
293RTV also stably expresses the SV40 large T antigen, through cloning and multiple rounds of testing for viral yield, 293RTV is specifically selected for high level of retroviral production. It offers several advantages over the regular 293T cells:
• High retroviral yield
• Firm attachment to culture plates and fast growing
• Ideal as a host when making retrovirus by cotransfection
Note: We recommend cotransfection of expression vector:gag-pol vector:envelope vector at the following plasmid ratios:
(a) For ecotropic or amphotropic retrovirus, 3:1:1
(b) For VSVG-pseudotyped retrovirus, 3:1:0.5
