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Cytoplasmic & Nuclear Extraction Kit
Cytoplasmic & Nuclear Extraction Kit
- 中文名称：
- Cytoplasmic & Nuclear Extraction Kit
- 英文名称：
- Cytoplasmic & Nuclear Extraction Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA45623
- 规格：
- 1 Kit|2x1 Kit|3x1 Kit|5x1 Kit|4x1 Kit
- 保存建议：
- Store at 4 degree C for one year.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: This kit provides a complete set of extraction reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and fresh tissues. The kit breaks cell membrane and release cytoplasmic proteins for cell burst under hypotonic condition. And then centrifuge for collection of the nucleoli. At last, extract nuclear proteins using Nuclear Extraction Reagent. Once desalted or diluted, the isolated soluble cytoplasmic proteins can be used to perform immunoassays and protein interaction experiments, such as EMSA, Co-IP and pull-down assays. Nuclear extracts are generally preferred to whole cell lysates for gene regulation studies. Cellular components present in whole cell lysates can adversely affect nuclear protein interactions and stability, and nuclear proteins are more concentrated in nuclear extracts than whole cell lysates.
