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Recombinant SARS-CoV-2 Spike protein, Alpha Variant of Concern, B.1.1.7 variant (United Kingdom, HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H)
Recombinant SARS-CoV-2 Spike protein, Alpha Variant of Concern, B.1.1.7 variant (United Kingdom, HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H)
- 中文名称：
- Recombinant SARS-CoV-2 Spike protein, Alpha Variant of Concern, B.1.1.7 variant (United Kingdom, HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H)
- 英文名称：
- Recombinant SARS-CoV-2 Spike protein, Alpha Variant of Concern, B.1.1.7 variant (United Kingdom, HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA47147
- 规格：
- 0.1 mg|2x0.1 mg|3x0.1 mg|5x0.1 mg|4x0.1 mg
- 保存建议：
- The protein can be stored at 2-8°C for 1 month. For long term, it should be stored at -20°C to -70°C preferably in small aliquots to avoid repeated freeze-thaw cycles.
- 货期：
- 6-8周
- 纯度：
- Affinity chromatography, >90% as estimated by SDS-PAGE
- 产品形式：
- Liquid; PBS 20% Glycerol
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Full-length soluble with foldon trimerization motif, mutated Furin recognition site and 4 stabilising mutations (A892P, A942P, K986P and V987P), based on/modified from Amanat et al, 2020 and Hsieh et al, 2020.
The first reports of atypical pneumonia in China were announced at the very end of 2019, in the city of Wuhan, Hubei province. Its cause was identified in January 2020 as a novel β-CoV (1), named SARS-CoV-2, which started spreading very quickly causing a global pandemic COVID-19. Spike protein attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (2, 3). The protein forms a trimer in vivo mediating receptor binding and membrane fusion (4). It has been demonstrated by Florian Krammer’s group that certain mutations and the inclusion of trimerization motif can stabilize recombinant Spike protein (5, 6). The original Wuhan strain of the virus has become quickly replaced by its more transmissible variant, mainly determined by a single amino acid point mutation D614G (7). The B.1.1.7 variant is a strain of the coronavirus with 8 distinctive mutations (in addition to the D614G) in the virus's spike protein and was first identified in autumn 2020 as the result of genetic sequencing of positive covid-19 samples by the Covid-19 Genomics UK Consortium (8).
上一篇：Recombinant SARS-CoV-2 Spike protein, Omicron variant, B.1.1.529, 21K clade
下一篇：Recombinant SARS-CoV-2 Spike protein, Delta B.1.617.2 variant (Indian variant, T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R and D950N)
