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Pyroptosis 660 Caspase-1 Kit
Pyroptosis 660 Caspase-1 Kit
- 中文名称：
- Pyroptosis 660 Caspase-1 Kit
- 英文名称：
- Pyroptosis 660 Caspase-1 Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA50395
- 规格：
- 25 Tests|2x25 Tests|3x25 Tests|4x25 Tests|5x25 Tests
- 保存建议：
- MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL.
Store the unopened kit (and each unopened component) according to the storage instructions on each component label. Store the Nigericin at -20 degree C.
Once reconstituted, the Nigericin stock should be used immediately or alliquoted and stored at -20 degree C for 12 months.
Avoid repeated freezing and thawing.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- 660 (Red Fluorescence)
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: Pyroptosis 660 Caspase-1 Kit utilizes the popular FLICA technology to detect caspase-1 activation. This kits contain the caspase-1 inhibitor reagent YVAD-FMK, which has the preferred binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD) (Chapman, 1992). This preferred caspase-1 binding sequence is labeled with 660 a far red fluorescent dye and linked to a fluoromethyl ketone (FMK) reactive entity. Caspase-1 will not cleave the FLICA inhibitor probe; instead, it forms an irreversible covalent bond with the FMK group on the reagent and becomes inhibited from further enzymatic activity.
Test Principle: To use FLICA, add directly to the cell culture medium, incubate, and wash. FLICA is cell-permeant and will efficiently diffuse in and out of all cells. If there is an active caspase-1 enzyme inside the cell, it will covalently bind with YVAD-FMK and retain the fluorescent signal within the cell. Unbound FLICA will diffuse out of the cell during the subsequent wash steps. Therefore, positive cells will retain a higher concentration of FLICA and fluoresce brighter than negative cells. There is no interference from pro-caspases or inactive forms of the enzymes. After labeling with FLICA, cells can be counter-stained with other reagents and fixed or frozen. Cells labeled with YVAD-FMK can be counter-stained with reagents such as the red live/dead stains Propidium Iodide and 7-AAD. Nuclear morphology may be concurrently observed using Hoechst 33342 (included in the kit), a blue DNA-binding dye. Cells can be viewed through a fluorescence microscope or flow cytometer.
