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8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose)
8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose)
- 中文名称：
- 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose)
- 英文名称：
- 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel (Rp-8-AHA-cAMPS-Agarose)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52690
- 规格：
- 0.6 mL|2.5 mL|6 mL|2x6 mL|3x6 mL
- 保存建议：
- 8-AHA-cAMPS agarose has sufficient stability for chromatography at room temperature and does not need special care during handling or shipment. Nevertheless for longer storing periods the gel should be kept in the refrigerator at 4 - 8°C since desulfurization yielding immobilized cAMP can occur slowly (about 0.1 % week/20°C). Storage buffer should contain 0.1 % sodium azide for prevention of microbial growth.
- 货期：
- 6-8周
- 纯度：
- The purity of each ligand to be attached is better than 98% (HPLC).
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
The PKA inhibitor Rp-cAMPS and the corresponding agonistic Sp-cAMPS are immobilized to agarose via a hexyl spacer at position 8 of the adenine nucleobase.
Properties of Rp-8-AHA-cAMPS Agarose: The ligand of this gel represents an immmobilized form of the cyclic AMP antagonist Rp-cAMPS. Free Rp-cAMPS was found to prevent dissociation of protein kinase A I & II catalytic from regulatory subunits and thus to inhibit the cAMP signal pathway. In addition Rp-cAMPS is not hydrolyzed by mammalian phosphodiesterases. Both properties make this analogue to be an interesting ligand in affinity chromatography, especially when trying to purify protein kinase A holoenzyme, cyclic nucleotide- dependent phosphodiesterases or other cAMP binding proteins. Immobilized Rp-cAMPS can also be used as a stable general ligand with reduced affinity for various nucleotide binding proteins since POE degradation, the first metabolic step necessary in cyclic nucleotide metabolism, is blocked. Immobilized Rp-cAMPS is also available with a short aminoethylamino spacer; for other spacer types, attachment positions or gels please inquire.
If the target protein is absorbed too strongly on common cAMP agaroses, both gels can used as alternative due to the reduced binding affinity of phosphorothioate-modified cyclic nucleotides to most receptors. Also, the column can be useful for purification of cGMP receptors in case the protein sticks too tightly to a corresponding gel with immobilized cGMP.
