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FAM-DEVD-OPH in vitro Apoptosis Detection Reagent, Pack of 4 Vials
FAM-DEVD-OPH in vitro Apoptosis Detection Reagent, Pack of 4 Vials
- 中文名称：
- FAM-DEVD-OPH in vitro Apoptosis Detection Reagent, Pack of 4 Vials
- 英文名称：
- FAM-DEVD-OPH in vitro Apoptosis Detection Reagent, Pack of 4 Vials
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52733
- 规格：
- 4 Vials|2x4 Vials|3x4 Vials|4x4 Vials|
- 保存建议：
- Store at 2-8 degree C
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: Detect apoptosis in cultured cell settings with the green fluorescent caspase 3/7 inhibitor reagent, FAM-DEVD-OPH. FAM-DEVD-OPH: 5-FAM-Asp-Glu-Val-Asp(OMe)-Oph (2,6-diF) caspase 3/7 inhibitor; Pack of 4 Vials.
Background: Apoptosis Detection with FAM-DEVD-OPH Caspase 3-7 Inhibitor Apoptosis is an evolutionarily conserved process of programmed cell suicide mediated by a cascade of proteolytic enzymes called caspases. Once activated in response to pro-apoptotic signals, caspases cleave protein substrates leading to the eventual disassembly of the cell1. Caspase Inhibitors with FMK Reactive Groups Early research studies using short peptide sequences linked to an aldehyde or fluoromethyl ketone (FMK) reactive group identified a number of peptide sequences preferentially targeted by different caspase enzymes. For example, the sequence Asp-Glu-Val-Asp (DEVD) is cleaved by activated caspase-3 and the sequence Val-Ala-Asp (VAD) demonstrates multiple caspase (activated caspase -1, -2, -3, -4, -5, -6, -7 -8, -9) recognition properties2. When linked to an FMK reactive group, the resulting peptide-FMK molecule will form an irreversible, covalent thioether adduct with the reactive SH-site of caspase enzymes. AAA Biotech utilized these findings to develop the often-cited FLICA (FLuorescence Labeled Inhibitors of CAspases) and FLIVO (FLuorescence in vIVO, now discontinued) caspase inhibitor and apoptosis detection reagents, both of which have been widely used for years as simple and reliable methods for screening apoptosis in live cells, tissues, and whole animals. These reagents consist of short peptide sequences with varying caspase specificities, a fluorescent label, such as carboxyfluorescein (FAM), and an FMK reactive moiety. The resulting fluorescent, cell-permeant, non-cytotoxic caspase inhibitor reagents can be added to cell culture media for whole cell apoptosis detection. These labeled caspase inhibitors permeate the cell membrane and form irreversible covalent bonds with activated caspase enzymes inside apoptotic cells, which can then be differentiated from non-apoptotic cells by their retained fluorescence. Caspase Inhibitors with O-phenoxy Reactive Groups AAA Biotech has now released a novel set of inhibitor reagents that employ an O-phenoxy (OPH) reactive group in place of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, the OPH caspase inhibitors form a stable, covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells3. OPH inhibitor compounds have the benefit of being extremely non-cytotoxic, display enhanced stability characteristics compared to the FMK analogs, and have been optimized to provide a high level of performance for in vitro applications3-4. The new FAM-OPH line of green fluorescent in vitro apoptosis detection reagents includes 5-FAM-Val-Ala-Asp(OMe)-2,6-difluorophenoxy-methyl ketone (FAM-VAD-OPH I) poly caspase inhibitor, 5-FAM-Val-Ala-Asp(OMe)-Oph (2,6-diF) (FAM-VAD-OPH II) poly caspase inhibitor, and 5-FAM-Asp-Glu-Val-Asp(OMe)-Oph (2,6-diF) (FAM-DEVD-OPH) caspase 3-7 inhibitor. A red fluorescent poly caspase inhibitor is also available: SR-VAD-OPH.
