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Staurosporine
Staurosporine
- 中文名称：
- Staurosporine
- 英文名称：
- Staurosporine
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52741
- 规格：
- 1 mg|2x1 mg|3x1 mg|5x1 mg|4x1 mg
- 保存建议：
- Store at -20°C. Avoid freeze/thaw cycles.
- 货期：
- 6-8周
- 纯度：
- >98%
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Staurosporine is a natural product derived alkaloid which is isolated from Streptomyces staurospores cultures. This protein kinase inhibitor belongs to a family of kinase inhibitors containing an indole carbozol chromophore. Staurosporine is a potent inhibitor of a number of kinases including: protein kinase C (PKC), cAMP dependent protein kinase, protein kinase A (PKA), tyrosine protein kinase, phosphorylase kinase, and Ca2+/calmodulan-dependent protein kinase. The ATP binding site on these kinase enzymes appears to be targeted by these Streptomyces derived inhibitors. Experiments using [3H]- staurosporine inhibition of purified PKC, PKA, tyrosine protein kinase, and Ca2+/calmodulan-dependent protein kinase enzymes revealed dissociation constants (KD) ranging from 2-10 nM. Human promyelocytic leukemia (HL-60) cells were found to be sensitive to apoptosis induction from a 2 hour exposure to staurosporine concentrations as low as 0.1 uM.
Inhibition of these intracellular kinases by staurosporine leads to the induction of apoptosis as exhibited by the classic chromatin condensation leading to the formation of micronuclear bodies and reduced cell volumes. DNA is subsequently cleaved into oligonucleosomal fragments (laddering) as evidenced by agarose gel analysis. Staurosporine was determined to be an effective apoptosis-inducing agent in virtually all cell lines that were exposed to this kinase inhibiting agent.
