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TMB 1-Component HRP Membrane Substrate
TMB 1-Component HRP Membrane Substrate
- 中文名称：
- TMB 1-Component HRP Membrane Substrate
- 英文名称：
- TMB 1-Component HRP Membrane Substrate
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA52745
- 规格：
- 100 mL|2x100 mL|3x100 mL|5x100 mL|4x100 mL
- 保存建议：
- Store at 2-8 degree C. Protect from light.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: TMB 1-Component HRP Membrane Substrate (SUBM) is suitable for membrane applications using horseradish peroxidase (HRP) as the conjugated detection enzyme. SUBM should not be used for microwell (ELISA) applications.
TMB 1-Component HRP Membrane Substrate is a one-component for-mulation containing stabilized 3,3’,5,5’-tetramethylbenzidine (TMB). The TMB substrate is oxidized by the peroxidase enzyme to yield an insoluble dark blue reaction product. SUBM is supplied ready-to-use at 1X.
Best results are obtained by equilibrating TMB 1-Component HRP Membrane Substrate to room temperature (25°C) prior to use. After probing with the antibody and HRP reagents, wash membrane thoroughly and transfer the membrane into a clean container. Cover the membrane surface with ample amount of SUBM and incubate 5-20 minutes. The substrate will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product.
For best results, monitor the substrate color development process until the target protein bands are visible. To stop the reaction, rinse the membrane with reagent quality water. If the reaction proceeds too long, there will be excessive background staining and diminished resolution of the target peptide or protein banding regions. If the color development is too rapid or intense, it is recommended to dilute the antibodies or conjugates or shorten the incubation period.
