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GoldHi EndoFree Plasmid Maxi Kit
GoldHi EndoFree Plasmid Maxi Kit
- 中文名称：
- GoldHi EndoFree Plasmid Maxi Kit
- 英文名称：
- GoldHi EndoFree Plasmid Maxi Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA55326
- 规格：
- INQUIRE
- 保存建议：
- 请按照说明书指示
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Endotoxin is a common contaminant in plasmid extraction. Because eukaryotic cells are very sensitive to endotoxin, the transfection efficiency will be greatly reduced if endotoxin is contained in the plasmid. This kit provides a simple, rapid and efficient method for the extraction of endotoxin-free plasmids. Based on conventional alkaline lysis method, the new and unique silicon membrane binds plasmid DNA efficiently and specifically. At the same time by using a special buffer system and endotoxin-removal filters, impurities such as endotoxin, genomic DNA, RNA, proteins are effectively removed.
100-300 ml of bacterial culture can be processed each time, and up to 2 mg of transfection-grade plasmid DNA can be obtained. The entire extraction process takes only 50 minutes. The plasmids obtained from the kit have high purity and high yield and are particularly suitable for transfection experiments. The plasmids can also be used for DNA sequencing, PCR, in vitro transcription, restriction endonuclease digestion and other experiments.
