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Enterokinase (Bovine) (His tag)
Enterokinase (Bovine) (His tag)
- 中文名称：
- Enterokinase (Bovine) (His tag)
- 英文名称：
- Enterokinase (Bovine) (His tag)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59047
- 规格：
- 100 Units|500 Units|2x500 Units|3x500 Units|4x500 Units
- 保存建议：
- Upon receipt, store at -80°C for long term.
Avoid repeated freeze and thaw cycles.
- 货期：
- 6-8周
- 纯度：
- > 95% by HPLC
- 产品形式：
- Lyophilized
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: Recombinant full length bovine Enterokinase with 6 His tags.
Introduction: Recombinant Enterokinase is the light chain of bovine Enterokinase, which is expressed in yeast and purified to yield a high enzyme activity preparation. Enterokinase recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. This enzyme can be used to cleave any fusion protein that carries this sequence. This product has higher cleavage activity than the native enzymes with 6 His tags which can be deleted after cleavage, and can also removed the rest enzymes rapidly without influencing the cleavage activity.
Background: Enterokinase (also called enteropeptidase, or protease serine 7, PRSS7) is an intestinal enzyme responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It is a serine protease consisting of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit.1 Enteropeptidase is a part of the chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.2 The gene catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
