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Anti-fade Mounting Medium (With DAPI)
Anti-fade Mounting Medium (With DAPI)
- 中文名称：
- Anti-fade Mounting Medium (With DAPI)
- 英文名称：
- Anti-fade Mounting Medium (With DAPI)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59111
- 规格：
- 10 mL|2x10 mL|5x10 mL|3x10 mL|4x10 mL
- 保存建议：
- Store at 2-8°C for one year
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Introduction:
1. With anti-fluorescence quenching reagent, this glycerol-based mounting medium has strong effect on anti-fade and can be used to seal tissues or cells slides in fluorescence related applications. After mounting, coverslipped slides will not readily dry out and can be observed for weeks afterwards without sealing (can be stored at 4°C or -20°C in dark for 2-3 weeks). The medium can prolong the duration of fluorescence and maintain the luminescence intensity of fluorescence maximally, in addition, the other components help to maintain the combination of antigen and antibody.
2. The medium are compatible with a wide array of fluorochromes, enzymatic substrates and fluorescent proteins, such as blue fluorescent protein, FITC, Cy2, Cy3, Cy5, Cy7, DAPI, Rhodamine, Texas Red, AMCA, R6G, Hoechst 33258, Alexa Fluor, etc.
3. DAPI (4',6-diamidino-2-phenylindole, C16H15N5, molecular weight: 350.25, CAS # 28718-90-3) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. DAPI can pass through an intact cell membrane therefore it can be used to stain both live and fixed cells.
4. DAPI is a blue-fluorescent DNA stain that exhibits 20-fold enhancement of fluorescence upon binding to A-T rich regions in DNA. It is excited by the violet laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry and chromosome staining. Because of its high affinity for DNA, it is also frequently used for counting cells, measuring apoptosis, sorting cells based on DNA content, and as a nuclear segmentation tool in high-content imaging analysis.
5. DAPI has an absorption maximum at a wavelength of 340 nm and its emission maximum is at 488 nm, when bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue).
