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Recombinant EZH2 protein complex
Recombinant EZH2 protein complex
- 中文名称：
- Recombinant EZH2 protein complex
- 英文名称：
- Recombinant EZH2 protein complex
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59636
- 规格：
- 0.025 mg|2x0.025 mg|3x0.025 mg|4x0.025 mg|5x0.025 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- 25ug recombinant EZH2 protein complex (contains EZH2, EED and Suz12 proteins) supplied in PBS (10mM Na2HPO4/KH2PO4), pH7.4, 2.7mM kCl, and 138mM NaCl.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: The EZH2 protein complex contains EZH2, EED and Suz12 which are the minimal components required for histone methyltransferase activity. EZH2 is specific for H3 lysine 27, a mark of repressed regions of chromatin. Recombinant human EZH2 (accession number NM_004456, amino acids 2-end with an N-terminal His-tag, molecular weight 86 kDa) was coexpressed in a baculovirus expression system along with human EED (accession number NM_003797, amino acids 2-end with an N-terminal DYKDDDDK tag, molecular weight 51 kDa) and human Suz12 (accession number NM_015355, amino acids 2-end with an N-terminal His-tag, molecular weight 87 kDa). EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the >mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers. Recombinant EZH2 protein complex can be used in histone methyltransferase (HMT) assays.
Background: EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2-(Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.
