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Recombinant SETD2 (1418-1714) protein
Recombinant SETD2 (1418-1714) protein
- 中文名称：
- Recombinant SETD2 (1418-1714) protein
- 英文名称：
- Recombinant SETD2 (1418-1714) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59638
- 规格：
- 0.05 mg|2x0.05 mg|3x0.05 mg|4x0.05 mg|5x0.05 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- 50ug recombinant SETD2 (1418-1714) expressed in E Coli and supplied in a buffer of 40mM Tris-HCl, pH8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 20% glycerol and 3mM DTT.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Set2 is a histone methyltransferase that methylates histone H3 at lysine 36. Amino acids 1418-1714 of Set2 (accession number NM_014159) were expressed in E Coli with an N-terminal GST-Tag (MW = 61 kDa). Recombinant Set2 can be used in histone methyltransferase (HMT) assays.
Background: SETD2 (SET Domain Containing 2) is a Histone methyltransferase that specifically trimethylates Lys-36 of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. SETD2 plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. SETD2 acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
