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Recombinant TRIM24 (862-980) protein
Recombinant TRIM24 (862-980) protein
- 中文名称：
- Recombinant TRIM24 (862-980) protein
- 英文名称：
- Recombinant TRIM24 (862-980) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59640
- 规格：
- 0.1 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >90% pure by SDS-PAGE.
- 产品形式：
- Recombinant TRIM24 (862-980) protein expressed in E Coli and supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: The peptide corresponding to amino acids 862-980 that contains the bromodomain sequences of TRIM24 (accession number NM_003852.3) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 18.8 kDa. It shows binding specificity for acetylated H3K9, H3K14 and H3K16. Tripartite motif-containing 24 (TRIM24) protein, also known as TIF1a, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM24 interacts with chromatin through recognition of specific histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at lysine 4 (H3K4me0) and acetylated at lysine 23 (H3K23ac). TRIM24 is an E3 ubiquitin-protein ligase that promotes proteosomal degradation of p53/TP53 and, in conjunction with TRIM33, mediates cell proliferation and apoptosis. TRIM24 also modulates transcriptional activation by retinoic acid (RA) receptors, including RARA, and has been shown to regulate RA-dependent proliferation of hepatocytes. TRIM24 functions as a transcriptional coactivator that interacts with the AF2 region of numerous nuclear receptors, including estrogen, RA and vitamin D3 receptors, and coactivators to modulate the transcription of target genes. It has been shown to be involved in upregulating ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Recombinant TRIM24 (862-980) can be used in binding assays and inhibitor screening.
Background: Tripartite motif-containing 24 (TRIM24) protein, also known as TIF1a, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM24 interacts with chromatin through recognition of specific histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at lysine 4 (H3K4me0) and acetylated at lysine 23 (H3K23ac). TRIM24 is an E3 ubiquitin-protein ligase that promotes proteosomal degradation of p53/TP53 and, in conjunction with TRIM33, mediates cell proliferation and apoptosis. TRIM24 also modulates transcriptional activation by retinoic acid (RA) receptors, including RARA, and has been shown to regulate RA-dependent proliferation of hepatocytes. TRIM24 functions as a transcriptional coactivator that interacts with the AF2 region of numerous nuclear receptors, including estrogen, RA and vitamin D3 receptors, and coactivators to modulate the transcription of target genes. It has been shown to be involved in upregulating ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1.
