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Recombinant p300 (1041-1161) protein
Recombinant p300 (1041-1161) protein
- 中文名称：
- Recombinant p300 (1041-1161) protein
- 英文名称：
- Recombinant p300 (1041-1161) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59642
- 规格：
- 0.1 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >90% pure by SDS-PAGE.
- 产品形式：
- Recombinant p300 (1041-1161) protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl, 5% Glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant p300 (1041-1161) protein was expressed in E Coli and contains an N-terminal His-Tag and C-terminal DYKDDDDK-Tag with an observed molecular weight of 19.1 kDa. This peptide, corresponding to amino acids 1041-1161, contains the bromodomain sequences of EP300 (accession number NM_001429.3). It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102. E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation, and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought to regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and it regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets.
Background: E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
