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Recombinant Tet1 (1418-2136) protein
Recombinant Tet1 (1418-2136) protein
- 中文名称：
- Recombinant Tet1 (1418-2136) protein
- 英文名称：
- Recombinant Tet1 (1418-2136) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59668
- 规格：
- 0.02 mg|2x0.02 mg|3x0.02 mg|4x0.02 mg|5x0.02 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The purity of the protein is >75% by SDS-PAGE.
- 产品形式：
- Recombinant TET1 (1418-2136) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant TET1 (1418-2136) protein that includes amino acids 1418-2136 of human TET1 (accession number NP_085128.2) was expressed in a baculovirus expression system and contains an N-terminal 6xHis-tag and DYKDDDDK-Tag with a molecular weight of 84.3 kDa. The purity of the protein is >75% by SDS-PAGE. It is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET1 preferentially binds to CpG-rich sequences at the promoters of both transcriptionally active and Polycomb-repressed genes and modulates gene silencing induced by cytosine methylation by controlling the levels of 5-mC and 5-hmC at gene promoters. TET1 may have the dual function of repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. TET1 is important for stem cell maintenance and specification and is involved in the balance between pluripotency and lineage commitment. TET1 plays an important role in regulating adult hippocampal neurogenesis and cognition
