-
Recombinant TRIM28 (624-811) protein
Recombinant TRIM28 (624-811) protein
- 中文名称：
- Recombinant TRIM28 (624-811) protein
- 英文名称：
- Recombinant TRIM28 (624-811) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59685
- 规格：
- 0.1 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >87% pure by SDS-PAGE.
- 产品形式：
- Recombinant TRIM28 (624-811) protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl, 5% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: The peptide corresponding to amino acids 624-811 that contains the bromodomain sequences of TRIM28 (accession number NP_005753.1) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 26.18 kDa. Tripartite motif-containing 28 (TRIM28) protein, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (Krüppel-associated protein), is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes enhances transcriptional repression by coordinating the increase in TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM28 has been shown to function as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). It also mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at lysine 9) to the promoter regions of KRAB target genes. TRIM28 enhances transcriptional repression by coordinating the increase in H3K9 methylation, the decrease in H3K9 and H3K14 acetylation, respectively, and the disposition of HP1 proteins to silence gene expression. It also acts as a corepressor for ErbB-4. TRIM28 inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation and may serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of pRb. TRIM28 has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. TRIM28 also specifically SUMOylates IRF7, inhibiting its transactivation activity, and ubiquitinates p53 leading to its proteosomal degradation. Recombinant TRIM28 (624-811) can be used in binding assays and inhibitor screening.
Background: Tripartite motif-containing 28 (TRIM28) protein, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (Krüppel-associated protein), is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes enhances transcriptional repression by coordinating the increase in TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM28 has been shown to function as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). It also mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at lysine 9) to the promoter regions of KRAB target genes. TRIM28 enhances transcriptional repression by coordinating the increase in H3K9 methylation, the decrease in H3K9 and H3K14 acetylation, respectively, and the disposition of HP1 proteins to silence gene expression. It also acts as a corepressor for ErbB-4. TRIM28 inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation and may serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of pRb. TRIM28 has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. TRIM28 also specifically SUMOylates IRF7, inhibiting its transactivation activity, and ubiquitinates p53 leading to its proteosomal degradation.
