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Recombinant KMT2C (MLL3) complex
Recombinant KMT2C (MLL3) complex
- 中文名称：
- Recombinant KMT2C (MLL3) complex
- 英文名称：
- Recombinant KMT2C (MLL3) complex
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59708
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >90% pure by SDS-PAGE.
- 产品形式：
- Full length Recombinant KMT2C (MLL3) complex expressed in E Coli cells and supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30) was generated using a peptide corresponding to amino acids 4740-4911 (accession number NP_733751.2) of the KMT2C protein that was expressed in E Coli. Recombinant KMT2C (MLL3) complex contains an N-terminal GST-Tag with a molecular weight of 46 kDa; human WDR5 (accession number NM_017588) that contains an N-terminal His-Tag with a molecular weight of 35 kDa; human ASH2L (accession number NM_001105214) that contains an N-terminal His-Tag with a molecular weight of 61 kDa; human RBBP5 (accession number NM_005057) that contains an N-terminal His-Tag with a molecular weight of 60 kDa; and human DPY30 (accession number NM_032574) that contains an N-terminal His-Tag with a molecular weight of 12 kDa. All Recombinant MLL3/WDR5/ASH2L/RBBP5/DPY30 complex proteins were individually expressed in an E Coli expression system. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: The KMT2C (MLL3) complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The KMT2C (also known as MLL3) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer.
