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Recombinant EZH1 complex
Recombinant EZH1 complex
- 中文名称：
- Recombinant EZH1 complex
- 英文名称：
- Recombinant EZH1 complex
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59721
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The recombinant protein is >95% pure by SDS-PAGE.
- 产品形式：
- Recombinant EZH1 complex was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant EZH1 complex contains full-length human EZH1 protein (accession number NP_001982.2) with N-terminal DYKDDDDK-Tag and MW = 86.3 kDa; full-length human SUZ12 (Accession No. NP_056170.2), with N-terminal 6xHis-tag and MW = 86.1 kDa; full-length human EED (Accession No. NP_003788.2), N-terminal 6xHis-tag and MW = 53.2 kDa; full-length human RbAp46 (Accession No. NP_001185648.1), N-terminal 6xHis-tag and MW = 55.3 kDa, and full-length human RbAp48 (Accession No. NP_005601.1), N-terminal 6xHis-tag and MW = 50.7 kDa, all individually expressed in Sf9 cells. The recombinant protein is >=95% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: EZH1 (Enhancer Of Zeste Homolog 1) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
