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Recombinant USP7 protein
Recombinant USP7 protein
- 中文名称：
- Recombinant USP7 protein
- 英文名称：
- Recombinant USP7 protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59733
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- The purity of USP7 protein is >80% by SDS-PAGE.
- 产品形式：
- Recombinant full length USP7 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 5% glycerol, 0.04% Triton X-100.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant human USP7 was expressed in a baculovirus expression system as the full-length protein (accession number NP_003461.2) with an N-terminal 6xHis-tag. The molecular weight of the protein is 131.3 kDa. The purity of USP7 protein is >80% by SDS-PAGE. It is suitable for analysis of enzyme function and transcription factor regulation as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. USP7 de-ubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, UHRF1, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. USP7 de-ubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. USP7 also de-ubiquitinates FOXO4 and inhibits FOXO4-induced transcriptional activity. Together with DAXX, USP7 is involved in the de-ubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 de-ubiquitinates UHRF1, which prevents its degradation and promoting DNA methylation by DNMT1.
