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Recombinant HDAC3/NCOR2 complex, His-Tag
Recombinant HDAC3/NCOR2 complex, His-Tag
- 中文名称：
- Recombinant HDAC3/NCOR2 complex, His-Tag
- 英文名称：
- Recombinant HDAC3/NCOR2 complex, His-Tag
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59781
- 规格：
- 0.05 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant HDAC3/NCOR2 Complex, His-tag is supplied in 50mM Tris-HCl pH8.0, 300mM NaCl, 5% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant HDAC3/NCOR2 complex, His-tag includes full-length human HDAC3 protein (accession number NP_003874.2) with a C-terminal 6xHis-tag and human NCOR2 amino acids 395-489 (accession number NP_006303.4) with an N-terminal GST-Tag; it was expressed in Sf9 cells. The molecular weights of HDAC3 and NCOR2 (395-489) are 49.7 kDa and 37.9 kDa, respectively. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: HDAC3 (Histone Deacetylase 3, also designated HD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressors NCOR2, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.
