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Recombinant IDH2 (R140K) protein
Recombinant IDH2 (R140K) protein
- 中文名称：
- Recombinant IDH2 (R140K) protein
- 英文名称：
- Recombinant IDH2 (R140K) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA59786
- 规格：
- 0.1 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant IDH2 (R140K) protein is supplied at a concentration of 0.5ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant IDH2 (R140K) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_002159.2) with a point mutation Arg140Lys and a C-terminal DYKDDDDK tag. The molecular weight of the protein is 51.7 kDa. It is suitable for assays in metabolism and pyruvate dehydrogenase complex as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Mutations in IDH1 and IDH2 frequently occur in grade II-III gliomas, secondary glioblastomas and acute myeloid leukemias (AML). To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG).
