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Recombinant YTHDC2 (1279-1429) protein
Recombinant YTHDC2 (1279-1429) protein
- 中文名称：
- Recombinant YTHDC2 (1279-1429) protein
- 英文名称：
- Recombinant YTHDC2 (1279-1429) protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA60134
- 规格：
- 0.1 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant YTHDC2 (1279-1429) protein is supplied in 25mM Tris- HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant YTHDC2 (1279-1429) protein corresponding to amino acids 1279-1429 that contains the YTH domain sequence of human YTHDC2 (accession number NP_073739.3) was expressed in E Coli with an N-terminal 6xHis-tag and a C-terminal DYKDDDDK tag. The molecular weight of the protein is 22.2 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.
