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Recombinant IKKe protein
Recombinant IKKe protein
- 中文名称：
- Recombinant IKKe protein
- 英文名称：
- Recombinant IKKe protein
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA60143
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant IKKe protein is supplied at a concentration of 0.3ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant human IKKe protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_054721.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 81.7 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IKKe (Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Epsilon, also known as IKBKE), is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. It's a serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. It also involves in TNFa and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, IKKe associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNb. In order to establish such an antiviral state, IKKe forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKKe-containing-complexes. It can be activated by polyubiquitination in response to TNF&alpha and interleukin-1, regulate the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. It can phosphorylate inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, IKKe is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at Ser-708. Phosphorylation of STAT1 at Ser- 708 seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses.
