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Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag
Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag
- 中文名称：
- Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag
- 英文名称：
- Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA60179
- 规格：
- 0.02 mg|1 mg|2x1 mg|3x1 mg|4x1 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag is supplied in 25mM HEPES pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Short Description: Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag was expressed in a baculovirus expression system as the full-length protein (accession number NP_071356.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 28.9 kDa.
Background: Prolyl Hydroxylase Domain-Containing Protein 3 (PHD3) or Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3), also known as PHIF-PH3 or HPH-3, is a prolyl hydroxylase. PHD3 is a cellular oxygen sensor that catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It can hydroxylate a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A, also HIF2A. PHD3 has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by PHD3 appears to require prior hydroxylation on the CODD site, and then hydroxylated HIFs are targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. PHD3 can also hydroxylate PKM in hypoxia, limiting glycolysis, while under normoxia, hydroxylate and regulates the stability of ADRB2. In cardiomyocytes, it inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, it has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. PHD3 is essential for hypoxic regulation of neutrophilic inflammation. Beside, it plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Its target proteins are preferentially recognized via a LXXLAP motif.
