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pA-Tn5 Transposase
pA-Tn5 Transposase
- 中文名称：
- pA-Tn5 Transposase
- 英文名称：
- pA-Tn5 Transposase
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA60348
- 规格：
- 0.01 mg|2x0.01 mg|3x0.01 mg|4x0.01 mg|5x0.01 mg
- 保存建议：
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Recombinant pA-Tn5 Transposase protein is supplied at a concentration of 0.3 µg/µl in 50 mM HEPES-KOH pH 7.4, 100 mM NaCl, 50% glycerol, 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT.
Recombinant pA-Tn5 Transposase protein was expressed in E.coli as the full length protein (accession number AAC25438.1) with an N-terminal 3×DYKDDDDK and protein A tag. The molecular weight of the protein is 75 kDa.
Transposase for transposon Tn5 or Tnp , also known as Tn5, Mediates transposition of transposon Tn5 by a ‘cut and paste’ mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).
