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TRANSIL Membrane Affinity Kit
TRANSIL Membrane Affinity Kit
- 中文名称：
- TRANSIL Membrane Affinity Kit
- 英文名称：
- TRANSIL Membrane Affinity Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA60728
- 规格：
- 8 Vials|2x8 Vials|3x8 Vials|4x8 Vials|5x8 Vials
- 保存建议：
- 储存在-20摄氏度
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Principle of the Assay: The principle of the TRANSIL Membrane Affinity kit is to assess the affinity of test compounds to phosphatidylcholine membranes. The membrane affinity is determined by incubating a fixed concentration of the drug candidate with varying concentrations of membrane surface area immobilized on the silica beads (Figure 1). The assay consists of 8 glass vials: Six vials contain membrane silica beads while two serve as references to account for non-specific binding and contain buffer only. Using the spreadsheet and algorithms supplied with the kit, the affinity of test compounds to the membrane is calculated from remaining free compound concentration in the supernatants. Any of the available detection systems, such as LC-MS/MS, scintillation counting, etc. can be used for quantification, as long as it can quantify uM concentrations in volumes of 200 ul or less.
