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SARS-CoV-2 Pseudoviral Particles, D614G-N501Y
SARS-CoV-2 Pseudoviral Particles, D614G-N501Y
- 中文名称：
- SARS-CoV-2 Pseudoviral Particles, D614G-N501Y
- 英文名称：
- SARS-CoV-2 Pseudoviral Particles, D614G-N501Y
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA62227
- 规格：
- 5 mL|2x5 mL|3x5 mL|4x5 mL|5x5 mL
- 保存建议：
- -70 C. Shipped on dry ice. Shelf Life: Six months from the date of shipping when store at -70 degree C
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- 10 ml (2 tubes, 5 mL/tube), for 2 multi-well plates
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
SARS-CoV-2 Pseudoviral Particles, replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein (the 614G-501Y variant).
The SARS-CoV-2 Pseudoviral Particles are replication-deficient Maloneymurine Leukemia Virus (MLV) pseudotyped with the SARS-CoV-2 spike protein of the 20E (EU1) variant carrying (Genbank Accession # QHD43416.1, p.501N-->Y, p.614D-->G).
It has been known that SARS coronavirus 2 (SARS-CoV-2) uses human ACE2 as the entry receptor and human proteases as the entry activators. The virus surface spike (S) protein mediates SARS-CoV-2 entry into cells. To fulfill its function, SARS-CoV-2 spike binds to the human ACE2 (hACE2) receptor through its receptor-binding domain (RBD) and then proteolytically activated by host proteases.
Our SARS-CoV-2 Pseudoviral Particles are replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein. They also contain the ORF for firefly luciferase as a reporter. They establish a pseudovirus entry assay for SARS-CoV-2 as the S protein mediated cell entry can be conveniently measured via the luciferase reporter activity. This pseudovirus assay isolates the SARS-CoV-2 viral entry from other steps of the viral infection cycle.
A new SARS-CoV-2 strain with an amino acid change at position 501 from Asn to Tyr besides the change at position 614 from Asp to Gly in the viral S protein predominated over time in locales where it was found. To support the study of this new SARS-CoV-2 strain, we established this SARS-CoV-2-614G-N501Y Pseudoviral Particles with the spike protein carrying the 501Y and 614G genotype (Genbank Accession #QHD43416.1, p.501 N->Y, p.614 D->G)
