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PDE4D, Active
PDE4D, Active
- 中文名称：
- PDE4D, Active
- 英文名称：
- PDE4D, Active
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA73102
- 规格：
- 0.01 mg|0.05 mg|0.5 mg|2x0.5 mg|3x0.5 mg
- 保存建议：
- Store product at –70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Ships on dry ice.
- 货期：
- 6-8周
- 纯度：
- >85%
- 产品形式：
- 50mM Tris-HCl, pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Description: Recombinant human PDE4D (225-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFalpha upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFalpha treatment and increased by 24 hours (1). Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility (2).
