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NAE1/UBA3 Complex, Active
NAE1/UBA3 Complex, Active
- 中文名称：
- NAE1/UBA3 Complex, Active
- 英文名称：
- NAE1/UBA3 Complex, Active
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA74077
- 规格：
- 0.02 mg|0.1 mg|1 mg|2x1 mg|3x1 mg
- 保存建议：
- Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- Recombinant protein complex is a combination of the following 2 buffer systems, adjusted to optimize the NAE1 (phosphate buffer system) and UBA3 (tris buffer system) proportions:
50mM sodium phosphate, pH7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Recombinant human NAE1 (2-end) and full-length human UBA3 were expressed by baculovirus in Sf9 insect cells. NAE1 used an N-terminal His tag, and the gene accession number is NM_003905. UBA3 used an N-terminal GST tag, and the gene accession number is BC022853.
NAE1/UBA3 is a heterodimeric E1 (Ub-activating)-like complex that activates NEDD8. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (3).
