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Ubiquitin Activating Enzyme E1 (human), (recombinant) (His-tag)
Ubiquitin Activating Enzyme E1 (human), (recombinant) (His-tag)
- 中文名称：
- Ubiquitin Activating Enzyme E1 (human), (recombinant) (His-tag)
- 英文名称：
- Ubiquitin Activating Enzyme E1 (human), (recombinant) (His-tag)
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA77053
- 规格：
- 0.05 mg|2x0.05 mg|3x0.05 mg|4x0.05 mg|5x0.05 mg
- 保存建议：
- For long term storage, store at -80°C. Avoid freeze/thaw cycles
Shipping: Shipped on Dry Ice
- 货期：
- 6-8周
- 纯度：
- >90% (SDS-PAGE)
Purified by multi-step chromatography
- 产品形式：
- Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1.0mM DTT.
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Ubiquitin activating enzyme E1 is responsible for the first step in ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating with ATP the C-terminal glycine carboxyl group of ubiquitin and, thereafter, linking this residue to the sulphydryl side chain moiety of a cysteine residue in E1 by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.
