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BrdU Immunohistochemistry Kit
BrdU Immunohistochemistry Kit
- 中文名称：
- BrdU Immunohistochemistry Kit
- 英文名称：
- BrdU Immunohistochemistry Kit
- 品牌：
- AAA Biotech
- 品牌介绍：
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具，核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号：
- AAA77708
- 规格：
- 50 Slides|2x50 Slides|3x50 Slides|4x50 Slides|5x50 Slides
- 保存建议：
- Upon receipt, store entire kit at -20oC. Once the kit is thawed, you may keep it at 4 degree C for 5 days. For long-term storage, it is recommended you aliquot and freeze the components at –20 degree C, particularly the Streptavidin-HRP Conjugate, the Prediluted Biotinylated Sheep anti-BrdU Detector Antibody and the 4x Trypsin Concentrate.
- 货期：
- 6-8周
- 纯度：
- N/A
- 产品形式：
- N/A
- 免责声明：
- *本产品仅供科研实验使用，不得用于临床诊断。*
- 说明书：
Background: A non-isotopic immunohistochemical staining procedure for the localization and quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. BrdU Immunohistochemistry Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and visual staining (dark brown nuclei) of these cells which is achieved using a Prediluted Biotinylated Sheep anti-BrdU Detector Antibody followed by Streptavidin-HRP Conjugate and DAB (diaminobenzidine) substrate.
Intended Uses: The BrdU Immunohistochemistry Kit is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
