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FLOW IntraCellular Staining Kit (IC FLOW)
FLOW IntraCellular Staining Kit (IC FLOW)
- 中文名称:
- FLOW IntraCellular Staining Kit (IC FLOW)
- 英文名称:
- FLOW IntraCellular Staining Kit (IC FLOW)
- 品牌:
- AAA Biotech
- 品牌介绍:
- AAA Biotech专注于为全球生命科学研究提供高品质的蛋白质研究工具,核心产品包括经严格验证的抗体、重组蛋白及ELISA试剂盒。
- 货号:
- AAA78396
- 规格:
- 1 Kit|2x1 Kit|3x1 Kit|4x1 Kit|5x1 Kit
- 保存建议:
- Store at 2-8 degree C
- 货期:
- 6-8周
- 纯度:
- N/A
- 产品形式:
- N/A
- 免责声明:
- *本产品仅供科研实验使用,不得用于临床诊断。*
- 说明书:
The Intracellular FLOW Staining Kit allows detection antibodies to access the intracellular protein in Flowcytometry analysis. This kit is specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and to increase fluorescence signaling. First, live cells can be fixed with the Fixation buffer, which cross-links the protein. Second, the 1X Permeabilization Buffer creates holes in the membrane, allowing the antibodies enter into the cells effectively. Washing steps and antibody dilutions are done using 1X Permeabilization Buffer. The final cell resuspension prior to analysis is done using The Staining Buffer. Permeabilization Buffer is supplied as a 10X solution. Prior to use, this should be diluted 10 fold in distilled water. (1ml of Permeabilization Buffer with 9 ml of distilled water). 10X Permeabilization buffer has natural tendency to precipitate, however, its function is not affected. 1X solution can be filtered.