
Anti-AIRE antibody (C-Term) (STJ70203)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.IHC: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5µg/ml.IF: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10µg/ml.
FC:Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

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