
Anti-GAPDH antibody (Internal) (STJ70842)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622, PMID: 29158223 and https://doi.org/10.1101/2022.01.21.477266, and in WB on Rat: PMID: 31087788 and 34584880.IHC: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2µg/ml.IF: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10µg/ml.

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