
Anti-Caspase 3 antibody (Internal) (STJ70944)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently observed in MOLT4 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.IF: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

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