
Anti-B7-H4 antibody (Internal) (STJ71250)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calculated MW of 30.9kDa according to NP_078902.2 isoform 1 and 20.7kDa according to NP_001240778.1 isoform 2 ). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.IF: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.
FC:Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

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