
Anti-PLA2G4A antibody (Internal) (STJ72270)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85-90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.IHC: Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/ml.IF: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.
FC:Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

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