
Anti-ANGPT1 antibody (Internal) (STJ72337)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:32000.WB: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged) , a band of approx. 48kDa was observed. No bands were observed in mock-transfected HEK293 and the 48kDa plus a 75kDa band was observed using anti-DYKDDDDK tag antibody.
An approx. 48kDa band was also observed in lysates of cell line K562 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IF: Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10µg/ml.FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

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