
Anti-Calcipressin-1 antibody (Internal) (STJ72487)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 40kDa band was observed in Human Cerebellum lysates (calculated MW of 31.9kDa according to NP_001272320.2). This molecular weight is observed by other commercial sources, and was blocked by incubation with the immunizing peptide. Recommended concentration: 1-1.5ug/ml. Primary incubation 1 hour at room temperature.Approx. 40kDa band corresponding to isoform 1L, was observed in Wild-type Mouse Brain lysates, which is not present in the KO mouse. Additional 55kDa bands were consistently observed in both the WT and KO Mouse, and are therefore a non-specific signal. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Data kindly provided by Dana Crawford, PhD, Albany Medical College, NY, USA.IF: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.
FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

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