
Anti-PPARGC1A antibody (Internal) (STJ73782)
克隆性:Polyclonal
浓度:0.5 mg/mL
稀释范围:Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx. 70+90kDa bands observed in lysates of cell line A431 and in nuclear HEK293 cell lysates, and an additional 85kDa band observed in HepG2 cell lysate (calculated MW of 91.0kDa according to NP_037393.1, 89.6kDa according to NP_001317681.1, and 77.1kDa according to NP_001317682.1). All bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1.5-3µg/ml. Primary incubation 1 hour at room temperature.IHC: Paraffin embedded Human Liver and Kidney. Recommended concentration: 5-6µg/ml.IF: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.
FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

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